Benefits
- RIN values ≥ 7 for most tissues
- Rapid RNase inactivation
- Best protection at 7 and 14 days at RT
- No rtPCR or library prep inhibition
- Ensures reliable RNAseq data
- Makes field collection of tissue samples easier
Figure 2.. Agilent TapeStation 4200 gel images of purified mouse tissue RNA samples after 7 and 14-day incubation at room temperature in RNAdvantage or RNAlater solution.
Virtually guaranteed excellent RNAseq results
University core sequencing facilities often have more stringent requirements on the RIN number for RNA being submitted for RNAseq. The RIN values for GenTegra RNAdvantage protected tissue samples even at 14-days of room temperature storage meet or exceed the requirements for commercial sequencing services such as Azenta (Genewiz0 RIN ≥6.0 and Novogene RIN ≥5.8. The RIN values for the RNAdvantage protected samples are either at or so close to the Core lab requirements as to ensure excellent RNAseq results.
Figure 3. In every tissue tested the RIN score at 7-days for RNAdvantage protected tissue exceeded the stringent requirement for a RIN score of ≥7 and in all tissues the 14-day RIN scores were such that excellent RNAseq could be performed on the samples.
Of equal importance with the RIN numbers is that there is no interference with downstream qPCR, etc. As can be seen in Figure 4, RNAdvantage and RNAlater show no significant impact on the expected Ct values when compared to untreated tissue samples.
Figure 4. Average Ct values for GAPDH-qPCR from purified mouse tissue RNA samples after 7-day incubation in RNAdvantage or RNAlater
28s and 18s Ratios The ratio of the 18s peak to the 28s peak is another metric for RNA integrity. Over an assortment of six different tissue types, the ratios for 28s/18s were consistently better for RNA tissue samples protected with RNAdvantage at 14 days then for RNAlater protected samples at 7 days (data available upon request). As an example, spleen tissue showed essentially no change between 7 days and 14 days at room temperature.
Total RNA Integrity
Figure 5. Agilent TapeStation 4200 electropherograms (EPGs) for six purified mouse RNA tissue samples after 7 and 14-day incubation at room temperature in RNAdvantage or RNAlater solution.
RNA Protection from Beginning to End™ GenTegra offers a suite of products for RNA protection:
- RNAdvantage for protecting RNA in fresh tissue samples
- RNAssure™ for purified RNA, providing protection as the RNA is eluted
- GenTegraRNA™ for safely shipping or storing RNA at room temperature