Virtually guaranteed excellent RNAseq results
University core sequencing facilities often have more stringent requirements on the RIN number for RNA being submitted for RNAseq. The RIN values for GenTegra RNAdvantage protected tissue samples even at 14-days of room temperature storage meet or exceed the requirements for commercial sequencing services such as Azenta (Genewiz0 RIN ≥6.0 and Novogene RIN ≥5.8. The RIN values for the RNAdvantage protected samples are either at or so close to the Core lab requirements as to ensure excellent RNAseq results.
28s and 18s Ratios The ratio of the 18s peak to the 28s peak is another metric for RNA integrity. Over an assortment of six different tissue types, the ratios for 28s/18s were consistently better for RNA tissue samples protected with RNAdvantage at 14 days then for RNAlater protected samples at 7 days (data available upon request). As an example, spleen tissue showed essentially no change between 7 days and 14 days at room temperature.